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Impact of primer dimers and self-amplifying hairpins on reverse transcription loop-mediated isothermal amplification detection of viral RNA - Analyst (RSC Publishing)
Life | Free Full-Text | Use and Misuse of Cq in qPCR Data Analysis and Reporting | HTML
Polymerase Chain Reaction (PCR) – Tips for successful PCR and Troubleshooting PCR-qPCR | abm Inc.
Real-time Quantitative PCR - Creative BioMart
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Primer Dimers | How Primer Dimers Are Formed | Primer Dimer Formation | - YouTube
Glen Report 21.11 - HOT START PCR update: CleanAmp™ Primers
IJMS | Free Full-Text | A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses | HTML
Fluorescence acquisition during hybridization phase in quantitative real-time PCR improves specificity and signal-to-noise ratio | BioTechniques
Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma | Virology Journal | Full Text
qPCR Assay Design and Optimization | Bio-Rad
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions | BMC Biotechnology | Full Text
Universal ProbeLibrary System Performance Data
Glen Report 21.11 - HOT START PCR update: CleanAmp™ Primers
Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers | BMC Biotechnology | Full Text
Adapter dimers: causes, effects, and how to remove them
Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR | Experimental & Molecular Medicine
IJMS | Free Full-Text | A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses | HTML
Selection and validation of reference genes by RT-qPCR for murine cementoblasts in mechanical loading experiments simulating orthodontic forces in vitro | Scientific Reports
Real-Time PCR Doctor | Gene Expression/Quantification Experiments | Bio-Rad
Getting Things Backwards to Prevent Primer Dimers - The Journal of Molecular Diagnostics
Basics concepts of molecular virology
Real time PCR training | The Specialty Training Center